Hi, we are hosted at a Data center where we have a core router only for PNI only.
We need to host looking glass there.
https://github.com/telephone/LookingGlass
Can we simply upload this to Microtik/Files and access it and get the results ?
No.
oh badluck 
You need a system that can run PHP. It can be as simple as a Raspberry Pi.
In theory it would be possible to run a Metarouter running Linux on some MikroTik models, but I think
support for it is dwindling and it is very hard to get it running and stable.
Be aware this looking glass only supports ping/trace/mtr, no BGP.
I’ve worked on a similar project a little while back to support Mikrotik Routers and Quagga.
https://github.com/Cha0sgr/BGP-Looking-Glass-NG
It’s heavily built for use in http://www.awmn.net network, but it can work without it.
It mainly supports connecting to Mikrotik routers and listing their BGP routes in cisco/quagga ‘sh ip bgp’ format, but it also supports traceroute via mikrotik.
It doesn’t support other features that https://github.com/telephone/LookingGlass has, but since the project is open source you are very welcome to add new features if you’d like 
Oh, and to answer your original question, not you cannot use any of these systems directly on mikrotik. You need a separate LAMP server.
how will a user see from frontend the ping and trace ?
I don’t understand the question.