Physical Links + Trunking

Does trunking require all interfaces in the trunk to be physically connected?

I have the CRS226-24G-2S+ set up with a single switch group with the SFP+ in a trunked configuration.

[admin@MikroTik] > interface ethernet export
/interface ethernet
set [ find default-name=ether1 ] name=ether1-master
set [ find default-name=ether2 ] master-port=ether1-master
set [ find default-name=ether3 ] master-port=ether1-master
....
set [ find default-name=ether21 ] master-port=ether1-master
set [ find default-name=ether22 ] master-port=ether1-master
set [ find default-name=sfp-sfpplus1 ] master-port=ether1-master
set [ find default-name=sfpplus2 ] master-port=ether1-master
/interface ethernet switch trunk
add member-ports=sfp-sfpplus1,sfpplus2 name=trunk1

I only have one fibre link physically connected and I am seeing some of the ethernet clients cannot reach devices over this trunked interface whilst others can.

[admin@MikroTik] > /interface ethernet cable-test sfp-sfpplus1,sfpplus2 once     
      name: sfp-sfpplus1 sfpplus2
    status: no-link      link-ok

The documentation states:

The Trunking in the Cloud Router Switches provides static link aggregation groups with hardware automatic failover and load balancing.

Which leads me to believe that I should not see issues with just a single physical link connected.