Port Forwarding Port 80 not working

Dear all Master.
Please help me.

I have a Web Server Local, with IP Address: 192.168.1.109
Web Server Port: 80
my IP Public: 180.249.138.xxx

On Mikrotik RB950, I do Port Forwarding like this:

/ip firewall nat add chain=dstnat dst-address=180.249.138.xxx protocol=tcp dst-port=80
action=dst-nat to-addresses=192.168.1.109 to-ports=80

After that, I tried to access the Web server at 180.249.138.xxx from the Internet. But always fails

I checked Port 80 at http://www.yougetsignal.com/tools/open-ports/
the Results: Port 80 is closed on 180.249.138.xxx

You have opened the port 80 in the firewall?