Routeren

ether1 = ISP ip range 192.168.0.0/24 gw 192.168.0.1
Ether2 = 192.168.100.1/24 DHCP server enabled.
I want this route without nat maskerading , can I?
How?

Sure. Configure those IPs on the interfaces: http://wiki.mikrotik.com/wiki/Manual:IP/Address

Configure the DHCP server: http://wiki.mikrotik.com/wiki/Manual:IP/DHCP_Server

Configure a default route: http://wiki.mikrotik.com/wiki/Manual:IP/Route

/ip route add dst-address=0.0.0.0/0 gateway=192.168.0.1

Don’t configure NAT.

The manual is linked in the forum header. Please read it - you are asking extremely fundamental questions.

I can’t still connect with internet.
i have the route add.
pc.jpg

Post the output of “/ip address print detail”, “/ip route print detail”, “/interface print”, “/ip firewall export”, and an accurate network diagram.
Wrap the output in

 tags to keep it readable.

This is what i have done,
no firewall rules, no forwarding settings
How take i prints of my settings?
route-list.jpg
interface-list.jpg
adress-list.jpg

Here my schema
schema.jpg

That looks fine.

CLI commands are run via the New Terminal button in winbox. You can copy/paste from there.

Are you sure you do not need NAT? Is a router further upstream going to NAT 192.168.100.0/24 for you? Do all routers further upstream have a route to 192.168.100.0/24 via your DHCP address? Usually DHCP interfaces do not play well with routing. One way to work around that is NAT, as the upstream router now sees directly connected IPs.
In short, I think you need to NAT.

/ip firewall nat add chain=srcnat out-interface=ether1-ISP action=masquerade

Thanks for your help again.