Hi Experts

When I Disable Use default gateway on remote network I cant ping my internal network. What do I need to change on Mikrotik?

Run command /ip route print with gateway setting enabled and disabled and see what is tge difference.

I have attach IP route print

I don't think Mikrotik (if that's your local router) has anything to do with it, it's your VPN which is greedy taking over all of traffic. Many VPN setups do it, excuse is that if a PC becomes member of another LAN (via VPN), it should not become potential bridge between both LANs. Depending on VPN client you're using you might not have any possibility to affect the behaviour.