Queue Tree - not work

everyone, i need help for my network queue… i`m a newbie person for mikrotik system
i need to limit bandwidth for streaming youtube video, but my setting queue not work.

this is my network topology:

this is my interface mikrotik:




This is my configuration :

firewall
layer7 protocol - name “streaming”:

^.+(c.youtube.com|cdn.dailymotion.com|metacafe.com |mccont,com).*$

Mangle Rule :

chain : prerouting
layer7 protocol : streaming
action : mark packet
new packet mark : streaming
passthrough : No

Queue

Name: streaming
parent : ether1-astinet
queue type : default
priority : 8
max limit : 32k

i need help, please for solution…